ИЗМЕНЕНИЕ ПЕРОКСИДАЗНОЙ АКТИВНОСТИ В ПРОРОСТКАХ ПОДСОЛНЕЧНИКА В УСЛОВИЯХ МОДЕЛИРОВАННОГО ВОДНОГО СТРЕССА

нжела РУДАКОВА, Ала КЕРДИВАРА, Сергей РУДАКОВ, Государственный Университет Молдовы

Rezumat

Total peroxidase activity was determined in leaves of 9 sunflower genotypes (Helianthus annuus L.) under the influence of drought artificially simulated using polyethyleneglycol (PEG). Electrophoretic profiles of peroxidase isoenzymes in control and experimental samples were also studied. The greatest increase in peroxidase activity under severe drought was demonstrated by two genotypes (H3 and RF3). When studying the electrophoretic spectra of peroxidase isoenzymes in the studied sunflower genotypes, 9 isoforms with molecular masses from 42 kD to 121 kD were identified. Under the influence of drought, various processes occurred in the isoenzyme composition of peroxidases in different genotypes: several zones were intensively accumulated, the content of another zone slightly decreased, and some zones were synthesized de novo. Consequently, under drought conditions, sunflower seedlings exhibited varying changes in peroxidase activity, including either the stimulation or inhibition of specific isoenzyme synthesis.

Keywords: peroxidase activity, isoenzymes, sunflower genotypes, drought, electrophoretic spectra.

DOI:https://doi.org/10.59295/sum6(186)2025_29