PROFILUL ACTIVITĂŢII CATALAZEI ŞI PRODUCERII DE PROTEINE LA SACCHAROMYCES CEREVISIAE CNMN-Y-20 SUB INFLUENŢA NANOPARTICULELOR ZnO

Agafia USATÎI, Natalia CHISELIŢA Institutul de Microbiologie şi Biotehnologie

Abstract

PROFILE OF THE CATALASE ACTIVITY AND PROTEIN PRODUCTION AT SACCHAROMYCES CEREVISIAE CNMN-Y-20 UNDER THE ZnO NANOPARTICLES INFLUENCE 

This work offers new data regarding the relations between the antioxidant enzyme activity – catalase and the para-meters of biomass production and proteins of Saccharomyces cerevisiae CNMN-Y-20 strain under the influence of 10, 30 and <100 nm ZnO nanoparticles. It was established, that the proteins amount and the antioxidant enzyme activity - catalase at the yeast strain at the cultivation in presence of ZnO nanoparticles has changed depending on dimensions and the ZnO nanoparticles concentrations. The 10 nm nanoparticles initiated a relative decrease of the protein quantity in the biomass, values observed at all applied concentrations. The 30 nm nanoparticles have given a positive effect only in the case when were used low concentrations of 1-5 mg/L. The obtained results with the application of nanoparticles with larger dimensions (<100 nm) indicated trends of increase of the protein amount in the yeast strain in contact with 0,5-1 mg/L concentrations, but the 5-15 mg/L concentrations decreased these values. 

The study regarding the relation between the indices of proteins amount and the catalase activity in Saccharomyces cerevisiae CNMN-Y-20 biomass in contact with ZnO nanoparticles revealed an average and low dependence in function of the nanoparticles dimensions. 

Thus, finishing this analysis we can assume that zinc oxide nanoparticles, penetrating into the cells cytoplasm, get in contact with cellular components, can determine an acceleration or a delay of biosynthetic processes. 

Keywords: Saccharomyces cerevisiae, ZnO nanoparticles, cell biomass, proteins, catalase.