СНИЖЕНИЕ АЛЛЕРГЕННОСТИ БЕЛКОВ НЕОЧИЩЕННЫХ ЭКСТРАКТОВ СЕМЯН АРАХИСА И СОИ МЕТОДОМ ОГРАНИЧЕННОГО ПРОТЕОЛИЗА

Алла КЕРДИВАРЭ, Андрей ШУТОВ Молдавский государственный университет

Abstract

LIMITED PROTEOLYSIS OF PROTEINS FROM NON-PURIFIED EXTRACTS OF PEANUT AND  SOYBEAN SEEDS REDUCES THEIR ALLERGENICITY 

Limited papain proteolysis of unpurified protein extracts from peanut and soybean seeds leads to cleavage off of the C-terminal region of the N-terminal domains of 11S globulins, as well as the N-terminal region of peanut 7S globulin. In these regions, IgE epitopes have been identified, largely determining the allergenicity of 11S and 7S peanut and soybean globulins. The results indicate the fundamental possibility of reducing the allergenicity of peanut and soybean proteins when using not only purified globulin preparations as substrates, as established earlier, but also total protein extracts of seeds. 

Keywords: seed storage globulins, allergens, IgE epitopes, peanut, soybean.