ОЦЕНКА ЭСТЕРАЗНОГО ПОЛИМОРФИЗМА В ЗРЕЛЫХ СЕМЕНАХ СУРЕПИЦЫ (Brassica campestris L.)

Анжела РУДАКОВА, Сергей РУДАКОВ, Анна АРТЕМЬЕВА*, Юрий ЧЕСНОКОВ** Молдавский государственный университет *ФГБНУ Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова, С.-Петербург, Россия **ФГБНУ Агрофизический научно-исследовательский институт, С.-Петербург, Россия

Abstract

EVALUATION OF ESTERASE POLYMORPHISM IN MATURE SEEDS OF COLESEED (Brassica campestris L.) 

To study the polymorphism by esterase composition 30 samples of mature seeds of various varieties and subspecies Brassica campestris L. from the VIR collection (St. Petersburg) were examined. 

The method of native electrophoresis in PAAG with the subsequent processing of the gels revealed 10 main zones of esterase enzymes, which were subdivided into 2 conditional loci – “b” and “c”. Five zones of higher molecular weight (42,0 кД – 32,4 кД) were designated as b1–b5. All of them turned out to be monomorphic, i.e. characteristic of all samples. The “c” locus was represented by five lower molecular weight zones (25,6 кД -15,0 кД ), which were designated as c1-c5. These zones were characterized by the highest polymorphism among the samples presented. 

According to their esterase complex, all samples were divided into 8 groups (zymotypes). The most common was the zymotype 1 (50% of the total number of genotypes). The second most common was zymotype 2, its representatives accounted for 16,7%. The maximum number of esterase enzymes (9 zones) were characterized by the zymotype 3 and 4. The zymotypes 7 and 8 have the smallest number of esterases (6 zones). 

The frequency of occurrence of each zone among all genotypes varied from 6,7% to 96,7%. The most common among all polymorphic esterase zones was the zone c3 (Mr 20,9 kD), the frequency of its occurrence was 96,7% of the total number of genotypes. 

The relative amount of different isoforms of esterase enzymes in samples varies significantly. The average statistical value of the content of all isoenzymes of locus “b” varies from 11,8% (zone b5) to 23,5% (zone b4), while for enzymes of the locus “c” this value varies from 2,2% (zone c1) to 7,1% (zone c3). 

Keywords: esterases, isozymes, electrophoresis, zymotype, genetic variability, locus, Brassica campestris.