STUDIUL ACŢIUNII MEDIILOR DE REHIDRATARE ÎN BAZA NANOPARTICULELOR ASUPRA VIABILITĂŢII MICROMICETELOR LIOFILIZATE

Tamara SÎRBU, Ion TIMUȘ, Olga ȚURCAN, Cristina MOLDOVAN, *Viorina GORINCIOI Institutul de Microbiologie și Biotehnologie * Institutul de Chimie

Abstract

STUDY OF THE ACTION OF REHYDRATION MEDIA ON THE BASIS OF NANOPARTICLES ON THE VIABILITY OF LYOPHILISED MICROMYCETES

The last important stage of lyophilisation is rehydration, which has a major influence on the viability of microorganisms. Various nutrient media, skim milk, distilled water, etc. are used as rehydration media for lyophilised micromycetes. In this study, distilled water and nanoparticle (NP) solutions Fe2O3 and Fe2ZnO4 in a concentration of (mg/l): 0.05; 0.1; 1.0 were used for the rehydration of lyophilised strains of micromycetes. The obtained results showed that the solutions of NP Fe2O3, and NP Fe2ZnO4, in a concentration of 0.1 mg/and 0.05 mg/l, had a different influence on the viability of micromycetes. In some strains of the genus Penicillium there was a stimulation of viability of 7-8%, and in others a decrease of 6-9%, compared to the control variant. The viability of strains of the genus Aspergillus and Trichoderma decreased by 5-10% when using NP at a concentration of 0.05 mg/l, and at a concentration of 0.1 mg/l varied within ± 2-3%, compared to the control variant. Keywords: micromycetes, rehydration medium, nanoparticles, lyophilisation, viability.