GENOTIPAREA POPULAȚIILOR DE OROBANCHE CUMANA Wallr. CU MARCHERI MICROSATELIȚI SSR

Maria DUCA, Ana MUTU, Steliana CLAPCO Universitatea de Stat din Moldova

Abstract

GENOTYPING OF OROBANCHE CUMANA WALLR. POPULATIONS WITH SSR MICROSATELLITE MARKERS The aim of this study was to evaluate the genetic diversity of 33 Orobanche cumana Wallr. populations with different geographical origin using 14 SSR microsatellite markers. The most of the markers (Ocum-052, Ocum-059, Ocum-070, Ocum-081, Ocum-087, Ocum-160, Ocum-196 and Ocum-197) a hight genetic diversity at the inter- and intra-population level, as well as regionally, have been highlighted. Within the broomrape populations investigated, the presence of 153 molecular-genetic profiles and 98 alleles was revealed. The length of the amplified SSR nucleotide sequences ranged from 76 to 343 bp. A high intrapopulation genetic variability for the populations from Taraclia, Svetlii, Izbiste, Cazanesti, Prepelita, Congaz (Republic of Moldova), Radnevo, Rosenova (Bulgaria), Hohott (China), Kesan, Merkez, Thrace (Turkey) and OR-SR43 (Serbia) was found. The populations of O. cumana from Bulgaria and Serbia proved to be relatively more homogeneous, according to the number of molecular profiles (1-3) and the average number of total alleles (3.67 – Bulgaria; 3.63 – Serbia), compared to those collected from the Republic of Moldova (1-10; 3.82, respectively), Turkey (1-6; 4.02, respectively) and China (1-9; 4.72, respectively). Keywords: Orobanche cumana Wallr., population, SSR markers, allele, genetic diversity, genetic fingerprint.