СМЕШАННЫЙ ТИП ПРОТЕОЛИЗА 11S ГЛОБУЛИНА КЕДРА ПАПАИНОМ

Наталия ЛАПТЕВА, Эллина МАКАЕВА, Сергей РУДАКОВ, Анжела РУДАКОВА, Ирина КАХОВСКАЯ, Андрей ШУТОВ НИЛ биохимии растений

Abstract

Limited and cooperative proteolyses of 11S globulin from Pinus sibirica seeds by papain were studied separately using SDS-electrophoresis and analysis of kinetics of the processes. The limited proteolysis consists of detachment of C-terminal segment from 11S globulin α-chains including helical domain. The cooperative process starts immediately from the very beginning of the reaction as a pseudo-first order reaction under unvarying rate constant. General regularities of proteolysis of P. sibirica and some other 11S globulins previously studied were compared.