ROLUL GLUCIDELOR ÎN STABILIZAREA STĂRII MORFOFUNCŢIONALE A SPERMEI DE COCOŞ ÎN PROCESUL DE CRIOCONSERVARE

Ion BALAN Institutul de Fiziologie şi Sanocreatologie al AŞM

Abstract

Carbohydrates are energy substrate for spermatozoa, which determine the need to include them in composition of the medium, both for hypothermic preservation as well as for super hypothermic of the sperm. The investigations undertaken in this paper had proposed to analyse physicochemical properties of carbohydrates on morphofunctional state of rooster spermatozoa in cryoconservation process of sperm. Experimental data have established that the inclusion of maltose in protective medium for rooster sperm contribute to maintain morphological state of spermatozoa and significantly reduces the concentration of their pathological forms. Stabilization of mobility, longevity and absolute index of rooster sperm survival is possible by using isotonic solutions of carbohydrates in the composition of synthetic medium for rooster sperm. The cryoconservation process of rooster sperm, diluted in cryoprotective medium, supplemented with solutions of nonelectrolyte in optimal concentration generate maintaining at a significant level of the functional indeces of rooster spermatozoa by using maltose and their decrease in case of use of lactose for this purpose.