EFECTUL UNOR IONI METALICI ȘI AGENȚI CHIMICI ASUPRA ACTIVITĂȚII FOSFATAZEI ACIDE IN VITRO DIN RĂDĂCINILE PLANTELOR PISUM SATIVUM L.

Maria CAUȘ, Universitatea de Stat din Moldova

Abstract

The aim of this study was to investigate the effects of some metals and chemical agents in vitro [5 mM] on the activity of acid phosphatase (AF) from the roots of Pisum sativum L. plants. The AF activity was determined using p-nitrophenyl phosphate as a substrate. It was shown that CoSO₄, CoCl₂, CaCl₂, MgSO₄, and CdCl₂ did not significantly modify the AF activity. Pb(NO₃)₂ showed a slight inhibition of ≈13%, while FeSO₄, KCl, and NaCl exerted a moderate inhibition of 28, 29, and 36%, respectively, on the AF activity. However, CuSO₄, MnSO₄, ZnSO₄, and ZnCl₂ demonstrated a more pronounced enzymatic inhibition of 41, 48, 56, and 63%, respectively. A more pronounced enzymatic inhibition (42–52%) was also observed for some phosphate ions. In vitro testing of specific inhibitors of AF showed that sodium orthovanadate, sodium molybdate, and ammonium molybdate almost completely inhibit AF.

Keywords: acid phosphatase, activity, metals, chemical agents, pea roots.

DOI:https://doi.org/10.59295/sum6(186)2025_21