ИЗОЗИМЫ ЭСТЕРАЗЫ ИНБРЕДНЫХ ЛИНИЙ И ГИБРИДОВ КУКУРУЗЫ

Андрей БАБИЦКИЙ Славянский университет, г. Кишинев, Молдова

Abstract

The separation of esterase isozymes from seedling of maize was carried out using standard disc PAGE technique. Significant differences were observed in esterase pattern in maize inbred lines and their hybrids. The esterase E8 enzyme, whose structural locus was demonstrated to be elsewhere in the genome, was referred as the mark for identification esterase isozymes on the gel. New highly sensitive method to detect the esterase by means of combination of indigo and tetrazolium dyes is developed. It has be found that the E4 esterase described before as being composed in series lines on the gel is really the artifact of interaction between cyclic hydroxamate molecules with lysine groups of this isozyme. Additive patterns of inheritance E4 esterase isozymes in hybrid produced from parental lines have been shown that the esterase E4 isozymes are the monomers. There has found the new way to split E8 esterase into monomers and it is prown as being a heterodimer. The substrate specificity and the dimer link by means of the sulphydryl groups can identify E8 esterase as acetylcholinesterase. 

Keywords: esterase, E4, E8 isozymes, acetylholinesterase, highly sensitive method to detect esterase, maize inbred lines and hybrids.