SINTEZA ORIENTATĂ A POLIZAHARIDELOR ACIDE LA CIANOBACTERIA SPIRULINA PLATENSIS CNM CB-02

Valentina BULIMAGA, Maria PISOVA, Liliana ZOSIM Universitatea de Stat din Moldova

Abstract

ACIDIC POLYSACCHARIDES DIRECTED SYNTHESIS AT CYANOBACTERIA SPIRULINA PLATENSIS CNM CB-02 

Two-stage cultivation of cyanobacterium Spirulina platensis CNM CB-02 with NaCl (0,25 and 0,50 M) supplemen-tation on the second stage of cultivation, without varying of the lighting regime ensured an increasing of the content of sulfated polysaccharides in biomass by 29 and 31,3%, respectively, compared to the reference sample (without NaCl). It wasn’t found an evident effect of NaCl on the total content of acidic polysaccharides in this case. Light intensity increasing (up to 5500 lx) with NaCl (0,25 M) supplementation ensured the increasing of the acidic polysaccharides content with 23,45%, compared to the reference sample (cultivated at 3500lx). CuSO4·5H2O (1-4 mg/l) hadn’t a significant effect on the content of acidic and sulfated polysaccharides in spirulina biomass, but considerably stimulated acidic exopoly-saccharides production (up to 238,3 g / kg) at 5500 lx. 

Keywords: spirulina, two-stage cultivation, stress factors, acidic intracellular polysaccharides and exopolysaccharides.