THERMOSTABILITY OF PEROXIDASE IN LEAVES OF BUXUS SEMPERVIRENS L. UNDER THE INFLUENCE OF HYPERTHERMIC SHOCK
Maria CAUȘ, Nicolai PLATOVSCHII, Moldova State University
Аннотация
The aim of this study was to investigate the thermal stability of total activity and peroxidase (PO) isoenzymes
isolated from boxwood Buxus sempervirens (L.) leaves subjected to hyperthermic shock (HTS) at temperatures in the
range of 53-90°C for 5 min. It was demonstrated that PO in leaves exposed to HTS of 53°C, 55°C and 57°C exhibits
high resistance to these temperatures, the activity being at the level of the control. Although, the total activity and
intensities of PO isoenzymes distributed in 4 zones in a vertical gradient (4-7%) of polyacrylamide gel (PAAG) gradually decreased with increasing HTS of temperatures above 58°C. However, treatment of leaves with HTS at 90°C
for 5 min resulted in complete suppression of PO, the activity of which was not detected.
Keywords: boxwood leaves, hyperthermic shock, activity, peroxidase, isozymes.
