ALERGENUL ARA H3, GLOBULINA DE REZERVĂ 11S DIN SEMINŢELE DE ARAHIDE 1. PROTEOLIZA LIMITATĂ CU PAPAINĂ

Ala CHERDIVARĂ, Angela RUDACOVA, Serghei RUDACOV, Andrei ŞUTOV Universitatea de Stat din Moldova

Аннотация

ALLERGEN ARA H3, STORAGE 11S GLOBULIN FROM PEANUT SEEDS

1. PAPAIN LIMITED PROTEOLYSIS

Papain proteolysis of the storage 11S globulin Ara h3 from peanut seeds starts from the detachment of an extended -chain C-terminal sequence covering the region of -helices. Further cleavage of a loop between -strands E’ and F’ inside the central part of -chain -barrel occurs generating fragments connected with intact -chains via a disulfide bond and those retained inside the molecule of partially hydrolyzed 11S globulin due to non-covalent interactions. In accordance with the described proteolysis scenario, the C-terminal -chain region containing three of the four antigen determinants (IgE epitopes) identified in the peanut 11S globulin is destroyed during the initial papain action. Therefore, the level of Ara h3 allergenicity can be decreased via papain limited proteolysis.

Keywords: seed 11S globulins, allergens, IgE epitopes, proteolysis, papain, peanut.